These figures help to confirm the likely roles for Asp 10 and 121, both of which are clearly essential for catalysis. This is in agreement with our proposal that they act as the activator of the catalytic nucleophile and the catalytic proton donor, respectively. The hydroxyl group of Tyr 8, https://israelefauo.mpeblog.com/44897951/die-besten-side-of-site-directed-mutagenesis