Secondly, it tunes the size with the overlap region iteratively in an endeavor to reduce the melting temperature variance of overlap. Thirdly, dynamic programming algorithm is used to accomplish the uniform melting temperature with the oligodeoxynucleotide overlaps. Lastly, the oligodeoxynucleotides with homologous melting temperature necessary for ligase chain response-based mostly https://gene-synthesis-companies09753.wiki-cms.com/6359474/facts_about_gene_synthesis_companies_revealed